توضیحات
Preparation
- Shake the container of Brucella Agar powder well.
- Dissolve 21 grams in 500 ml of distilled water,
- autoclave at 121°C for 15 minutes, and pour into plates.
- For Brucella differential agar, adjust the pH to 6.7 ± 0.1, sterilize Brucella agar, cool to 50°C. Introduce 1 ml of a 2% basic fuchsin solution or prepare a 2% solution from thionine acetate, boiling the solution for 20 minutes.
- In the case of Brucella selective agar, sterilize Brucella agar, cool to 50°C. supplement the medium with the following compounds per 500 ml: Bacitracin 250 mg, Polymyxin B sulfate 0.5 mg, Cycloheximide 50 mg, and, if needed, ethyl violet 0.61 mg.
- For the isolation of Campylobacter species, cool the media to 50°C. Add a sterile, filtered solution containing 125 mg sodium pyruvate, 125 mg sodium metabisulfite, 125 mg ferrous sulfate in 2 ml of distilled water, along with 5-7% sterile defibrinated sheep blood. Additionally, add 25 ml heat-inactivated horse serum to 500 ml of the medium base.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
