توضیحات
Preparation
- Shake the container of Brucella Broth powder well.
- Suspend 28.1 g of powder in distilled water. Mix well.
- Autoclave at 121°C for 15 minutes.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
Quality Control
Quality Control
- Dehydrated Appearance: Fine, homogeneous, free of extraneous material.
- Prepared Appearance: Light to medium, tan to yellow, clear to slightly hazy.
- Cultural response after incubation at 35 ± 2°C for 7 days with 3 – 5% CO2 (incubate S. pyogenes for 66 – 72 hours without CO2).
*Minimally one strain of Brucella should be used for performance testing. If these strains are not available, verify performance with a known isolate.
Recovery |
Organism (ATCC) |
Growth
|
Brucella abortus (11192*)
|
Growth
|
Brucella melitensis (4309*)
|
Growth
|
Brucella suis (4314*)
|
Growth
|
Streptococcus pyogenes (19615)
|