Preparation
- Shake the container of cooked meat medium well.
- Suspend 12.5 g of the particles in 100 mL purified water.
- Let stand until all particles are thoroughly wetted and form an even suspension.
- Autoclave at 121°C for 15 minutes. Reduce pressure slowly and cool without agitation.
- If not used within 24 hours, reheat (100°C) prior to use to drive off absorbed oxygen.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.