Fraser Listeria Selective Enrichment Broth Base

Fraser Listeria Selective Enrichment Broth Base

Application: Used as a secondary selective diagnostic enrichment medium for the isolation of Listeria spp.

Industry: Food / Clinical

Catalogue Number: i23274

توضیحات

Preparation

Sake the container of Fraser Broth well.
Suspend 55 g in 1 L of distilled water,
sterilize by autoclaving at 121°C for 15 minutes,
cool to 50°C, and aseptically add Fraser Selective Supplement, including 0.5 g ferric ammonium citrate, 20 mg Nalidixic acid, and 20 mg Acriflavine hydrochloride. Mix well.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

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Components

gr / L	Components
5	Proteose peptone
5	Peptone from Casein
5	Yeast Extract
5	Meat Extract
20	Sodium Chloride
12	Di-sodium hydrogen phosphate
1.35	Potassium dihydrogen phosphate
1	Esculin
3	Lithium Chloride
0.2 ± 7.2	Final pH at 25°C

Quality Control

Dehydrated Appearance: Free flowing, homogeneous, tan.
Prepared Appearance: Medium amber, clear to slightly opalescent with a fine precipitate.
1- Enrichment step: The half-concentrated FRASER broth is inoculated with sample material and incubated at 30 °C for 24 ± 2 hours. From this culture, a selective growth medium such as OXFORD or PALCAM Agar is inoculated.
2- Enrichment step: From the first enrichment step, 0.1 ml is inoculated on to 10 ml FRASER broth for two incubations of 48 ± 2 hours at 35 °C or 37°C. After each 24 hours period selective growth media such as OXFORD and/or PALCAM agar are inoculated.

Esculin reaction	Recovery	Organism (ATCC)
+	+	Listeria monocytogenes (19111)
+	+	Listeria monocytogenes (13932)
-	Marked to complete inhibition	Escherichia coli (25922)
-	Marked to complete inhibition	Enterococcus faecalis (19433)
-	Marked to complete inhibition	Staphylococcus aureus (25923)

توضیحات

Preparation

Sake the container of Fraser Broth well.
Suspend 55 g in 1 L of distilled water,
sterilize by autoclaving at 121°C for 15 minutes,
cool to 50°C, and aseptically add Fraser Selective Supplement, including 0.5 g ferric ammonium citrate, 20 mg Nalidixic acid, and 20 mg Acriflavine hydrochloride. Mix well.

STORAGE

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr / L	Components
5	Proteose peptone
5	Peptone from Casein
5	Yeast Extract
5	Meat Extract
20	Sodium Chloride
12	Di-sodium hydrogen phosphate
1.35	Potassium dihydrogen phosphate
1	Esculin
3	Lithium Chloride
0.2 ± 7.2	Final pH at 25°C

Quality Control

Dehydrated Appearance: Free flowing, homogeneous, tan.
Prepared Appearance: Medium amber, clear to slightly opalescent with a fine precipitate.
1- Enrichment step: The half-concentrated FRASER broth is inoculated with sample material and incubated at 30 °C for 24 ± 2 hours. From this culture, a selective growth medium such as OXFORD or PALCAM Agar is inoculated.
2- Enrichment step: From the first enrichment step, 0.1 ml is inoculated on to 10 ml FRASER broth for two incubations of 48 ± 2 hours at 35 °C or 37°C. After each 24 hours period selective growth media such as OXFORD and/or PALCAM agar are inoculated.

Esculin reaction	Recovery	Organism (ATCC)
+	+	Listeria monocytogenes (19111)
+	+	Listeria monocytogenes (13932)
-	Marked to complete inhibition	Escherichia coli (25922)
-	Marked to complete inhibition	Enterococcus faecalis (19433)
-	Marked to complete inhibition	Staphylococcus aureus (25923)

Fraser Listeria Selective Enrichment Broth Base

Preparation

STORAGE

Quality Control

Preparation

STORAGE

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

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CoA & MSDS files

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