Preparation
- Shake the container of Kligler Iron Agar well.
- Suspend 55 g in 1 L of distilled water,
- Autoclave for 15 min. at 121°C.
- Cool in a slanted position such that deep butts are formed.
- For best results, the medium should be used on the date of preparation or melted and resolidified before use.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.