توضیحات
Preparation
- Shake the container of Nitrate Broth well.
- Suspend 9 g of the medium in 1 L of distilled water and Mix well.
- Autoclave at 121°C for 15 minutes.
Preparation of Reagents
- Reagent A, a sulfanilic acid solution, is prepared by dissolving 8 g of sulfanilic acid in 1 liter of 5N acetic acid. This solution is stored at room temperature for up to 3 months in darkness, using dark glass containers or aluminum foil-wrapped bottles.
- Reagent B, an α-Naphthylamine solution, is created by dissolving 6 g of N,N-Dimethyl-1-naphthylamine in 1 liter of 5N acetic acid. It is stored in darkness at 2 to 8°C for up to 3 months using similar light-protective measures.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
