توضیحات
Preparation
- Shake the container of OF Basal Medium well.
- Suspend 9.4 g of the powder in 1 L of purified water. Mix well.
- Autoclave at 121°C for 15 minutes.
- Add 1% carbohydrate before or after autoclaving depending on heat lability.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
Quality Control
Quality Control
- Dehydrated Appearance: Light beige with green tinge, free-flowing, homogeneous.
- Prepared Appearance: Green, clear to very slightly opalescent.
- Prepare the medium without (plain) and with 1% dextrose. Inoculate tubes in duplicate with fresh cultures using an inoculating needle and add an overlay of mineral oil to one set of tubes. Incubate at 35 ± 2°C for 18 – 48 hours.
A**: Acid reaction, color changes to yellow.
K***: alkaline reaction, green medium.
AG****: Acid reaction, color changes to yellow and gas production.
|
With dextrose closed |
With dextrose open |
Plain closed |
Plain open |
Organism (ATCC) |
|
K
|
A**
|
K
|
K***
|
Acinetobacter Calcoaceticus (19606)
|
|
A, G
|
A, G****
|
K
|
K
|
Enterobacter Aerogenes (13048)
|
|
A, G
|
A, G
|
K
|
K
|
Escherichia coli (25922)
|
|
K
|
A
|
K
|
K
|
Pseudomonas aeruginosa (27853)
|
