Preparation
- Shake the container of PE-2 Broth well.
- Suspend 23 g in 1 litre distilled water.
- Dispense 18 – 20 ml aliquots into 18x150mm screw capped test tubes.
- Add 8 – 10 untreated Alaska seed peas and let the tubes stand for 1 hour to effect hydration.
- Autoclave at 121°C for 15 minutes.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.