Preparation
- Shake the container of TCBS Agar well.
- Suspend 89 g of the powder in 1 L of distilled water.
- Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
- Cool to 45 – 50°C. and use immediately.
- DO NOT AUTOCLAVE.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.