Preparation
- Shake the container of Tryptose Sulfite Cycloserine (TSC) Agar well.
- Suspend 42 g of powder in 1 L of distilled water.
- Autoclave at 121°C for 15 minutes.
- Cool the liquefied culture medium base to approx. 50 °C, add 0.4 g cycloserine (10 ml of filter-sterilized 5 % solution).
- If desired add 25 ml egg yolk emulsion (Not indicated in ISO).
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.