Tryptose Sulfite Cycloserine Agar

TSC Agar

Application: For the isolation and enumeration of the vegetative and spore forms of Clostridium perfringens in foodstuffs, clinical specimens and other materials.

Industry: Water / Food

Catalogue Number: i23229

توضیحات

Preparation

  • Shake the container of Tryptose Sulfite Cycloserine (TSC) Agar well.
  • Suspend 42 g of powder in 1 L of distilled water.
  • Autoclave at 121°C for 15 minutes.
  • Cool the liquefied culture medium base to approx. 50 °C, add 0.4 g cycloserine (10 ml of filter-sterilized 5 % solution).
  • If desired add 25 ml egg yolk emulsion (Not indicated in ISO).

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

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Quality Control

Quality Control

  • Dehydrated Appearance: Beige, powder, Homogenous
  • Prepared Appearance: Canary yellow, without significant precipitation.
  • After adding cycloserine supplement, cultural response was observed after incubation at 35 – 37 ˚C for 18 – 48 hours in anaerobic condition.
Black Colony Recovery Organism (ATCC)
+
Very good
Clostridium perfringens (12919)
-
Non/fair
Escherichia coli (25922)
Components
gr / L Components
15
Tryptose
5
Peptone from Soymeal
5
Yeast Extract
1
Sodium disulfite
1
Ferric Ammonium Citrate
15
Agar
0.2 ± 7.6
Final pH at 25°C
توضیحات

Preparation

  • Shake the container of Tryptose Sulfite Cycloserine (TSC) Agar well.
  • Suspend 42 g of powder in 1 L of distilled water.
  • Autoclave at 121°C for 15 minutes.
  • Cool the liquefied culture medium base to approx. 50 °C, add 0.4 g cycloserine (10 ml of filter-sterilized 5 % solution).
  • If desired add 25 ml egg yolk emulsion (Not indicated in ISO).

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Quality Control

  • Dehydrated Appearance: Beige, powder, Homogenous
  • Prepared Appearance: Canary yellow, without significant precipitation.
  • After adding cycloserine supplement, cultural response was observed after incubation at 35 – 37 ˚C for 18 – 48 hours in anaerobic condition.
Black Colony Recovery Organism (ATCC)
+
Very good
Clostridium perfringens (12919)
-
Non/fair
Escherichia coli (25922)
Components
gr / L Components
15
Tryptose
5
Peptone from Soymeal
5
Yeast Extract
1
Sodium disulfite
1
Ferric Ammonium Citrate
15
Agar
0.2 ± 7.6
Final pH at 25°C

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen