Preparation
- Shake the container of YM Broth well.
- Suspend 21 g in 1 liter of distilled water and Mix well.
- Autoclave at 121°C for 15 minutes.
- To increase selectivity, acidify the medium to pH 3.0 to 4.0 . by adding sterile 10% HCl, tartaric acid or 10% citric acid (penicillin 20 units per mL final concentration or streptomycin 40 μμg per mL final concentration) using aseptic technique
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.