Tryptone Water-Prepared Culture Media-ibresco

Tryptone Water (TW)

For cultivating non-fastidious organisms, for studying carbohydrate fermentation patterns and for performing the indole test.

Catalogue Number: i23185

توضیحات

Preparation

Shake the container well to homogenize the powder. Weigh 15 grams of the powder and dissolve it in 1 liter of distilled water.
Warm slightly with frequent agitation to completely dissolve the powder.
Autoclave at 121°C for 15 minutes.

For Determining Carbohydrate Fermentation Patterns

Add 1.8 milliliters of a 1% Phenol Red solution (i880137) to one liter of Tryptone Water. Mix well.
Dispense into test tubes containing inverted Durham vials. .
Autoclave at 121°C for 15 minutes.
Aseptically add sufficient sterile carbohydrate solution to yield a 1% final concentration. Rotate each tube to thoroughly distribute the carbohydrate.

STORAGE

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/Lit	Components
10	Peptone
5	Sodium Chloride
0.2 ± 7.3	pH

Quality Control

Dehydrated Appearance: White to light beige, free flowing, homogeneous.
Prepared Appearance: Light to medium amber, clear to slightly opalescent.
Prepare the medium per label directions. Inoculate and incubate at 35 ± 2°C for 18-24 hours. Add 0.5 mL Indole Reagent (Kovacs) to the tubes to test for indole production. Formation of a red color denotes a positive indole test.

Indole Production	Recovery	ATCC	Organism
+	Good	25922	Escherichia coli
-	Good	13047	Enterobacter cloacae

توضیحات

Preparation

Shake the container well to homogenize the powder. Weigh 15 grams of the powder and dissolve it in 1 liter of distilled water.
Warm slightly with frequent agitation to completely dissolve the powder.
Autoclave at 121°C for 15 minutes.

For Determining Carbohydrate Fermentation Patterns

Add 1.8 milliliters of a 1% Phenol Red solution (i880137) to one liter of Tryptone Water. Mix well.
Dispense into test tubes containing inverted Durham vials. .
Autoclave at 121°C for 15 minutes.
Aseptically add sufficient sterile carbohydrate solution to yield a 1% final concentration. Rotate each tube to thoroughly distribute the carbohydrate.

STORAGE

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/Lit	Components
10	Peptone
5	Sodium Chloride
0.2 ± 7.3	pH

Quality Control

Dehydrated Appearance: White to light beige, free flowing, homogeneous.
Prepared Appearance: Light to medium amber, clear to slightly opalescent.
Prepare the medium per label directions. Inoculate and incubate at 35 ± 2°C for 18-24 hours. Add 0.5 mL Indole Reagent (Kovacs) to the tubes to test for indole production. Formation of a red color denotes a positive indole test.

Indole Production	Recovery	ATCC	Organism
+	Good	25922	Escherichia coli
-	Good	13047	Enterobacter cloacae

Tryptone Water (TW)

Preparation

For Determining Carbohydrate Fermentation Patterns

STORAGE

Quality Control

Preparation

For Determining Carbohydrate Fermentation Patterns

STORAGE

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

Downloading CoA & MSDS files

CoA & MSDS files

Downloading CoA & MSDS files