توضیحات
Preparation
Broth Base
- Shake the container well to homogenize the powder. Suspend 8.4 g (single-strength broth) or 25.1 g (triple-strength broth) in 1 liter of demineralized water.
- Dispense in 50 ml volumes into suitable culture vessels and autoclave (15 min at 121°C).
- The broth base is clear and yellow-brown.
Malachite-green solution
- Weigh 0.15 grams of malachite green oxalate (i880109) and dissolve it in 90 milliliters of distilled water.
- sterilize by filtration.
Complete broth
- Add to 50 ml of cooled base broth 0.3 ml (single-strength broth) or 0.9 ml (triple-strength broth) of the malachite-green solution under sterile conditions.
STORAGE
Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.
