Simmons citrate-P. aeruginosa (27853)-ibresco

Simmons citrate-Prepared Media Plate-ibresco

SIMMONS Citrate Agar

Application: For the differentiation of Gram-negative bacteria on the basis of citrate utilization. Bacteria that can utilize citrate as sole carbon source turn the medium blue.

Catalogue Number: i23171

توضیحات

Preparation

Shake the container well to homogenize the powder. Suspend 22.3 g of the powder in 1 L of purified water. Mix thoroughly. Before using distilled water, check the pH level of the water to be between 6.5-7. If this point is not considered, the original color of the medium may be different.
Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
Autoclave at 121°C for 15 minutes.
Allow to cool in a slanted position for use as slants. The agar also may be used as a plating medium.

STORAGE

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

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Components

gr/Lit	Components
5	Sodium Chloride
2	Sodium Citrate
1	Di Potassium Hydrogen Phosphate
1	Ammonium Di Hydrogen Phosphate
0.2	Magnesium Sulphate.7H2O
0.08	Bromothymol Blue
13	Agar
0.2 ± 6.6	pH

Quality Control

Dehydrated Appearance: Light orange free-flowing, homogeneous may contain many dark and gray flecks.
Prepared Appearance: Medium to dark, green, clear to slightly hazy, with a small amount of precipitate and as many as a large amount of insolubles.
Cultural Response after inoculation with fresh cultures and incubate aerobically at 35 ± 2°C for 24-48 hours.

Color change to blue	Recovery	ATCC	Organism
-	None/poor	25922	Escherichia coli
Alkaline (blue)	Good	13047	Enterobacter cloacae
Alkaline (blue)	Good	8090	Citrobacter freundii
-	None/poor	12022	Shigella flexneri

توضیحات

Preparation

Shake the container well to homogenize the powder. Suspend 22.3 g of the powder in 1 L of purified water. Mix thoroughly. Before using distilled water, check the pH level of the water to be between 6.5-7. If this point is not considered, the original color of the medium may be different.
Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
Autoclave at 121°C for 15 minutes.
Allow to cool in a slanted position for use as slants. The agar also may be used as a plating medium.

STORAGE

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/Lit	Components
5	Sodium Chloride
2	Sodium Citrate
1	Di Potassium Hydrogen Phosphate
1	Ammonium Di Hydrogen Phosphate
0.2	Magnesium Sulphate.7H2O
0.08	Bromothymol Blue
13	Agar
0.2 ± 6.6	pH

Quality Control

Dehydrated Appearance: Light orange free-flowing, homogeneous may contain many dark and gray flecks.
Prepared Appearance: Medium to dark, green, clear to slightly hazy, with a small amount of precipitate and as many as a large amount of insolubles.
Cultural Response after inoculation with fresh cultures and incubate aerobically at 35 ± 2°C for 24-48 hours.

Color change to blue	Recovery	ATCC	Organism
-	None/poor	25922	Escherichia coli
Alkaline (blue)	Good	13047	Enterobacter cloacae
Alkaline (blue)	Good	8090	Citrobacter freundii
-	None/poor	12022	Shigella flexneri

SIMMONS Citrate Agar

Preparation

STORAGE

Quality Control

Preparation

STORAGE

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

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CoA & MSDS files

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