Mitis Salivarius Agar-E. faecalis (29212)-ibresco

Mitis Salivarius Agar

Application: For the isolation of Streptococcus mitis, Streptococcus salivarius, and Enterococci, particularly from heavily contaminated samples, use a 1% potassium tellurite (iS47083) solution.

Catalogue Number: i23384

توضیحات

Preparation

Shake the container well to homogenize the powder. Suspend 90 g of the powder in 1 L of purified water.
Autoclave at 121°C for 15 minutes.
Let the medium cool to a temperature of 55-50 degrees Celsius, then add 1 milliliter of 1% potassium tellurite solution (iS47083).
After adding potassium tellurite solution, do not heat the medium under any circumstances.

STORAGE

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

Test

Immediately after reaching the laboratory, inoculate the sample on the medium.
Incubate the plates at 35 ± 2 °C for 24-48 hours and 5% carbon dioxide.
Also culture a non-selective plate (such as blood agar) from the same sample. This increases the chance of recovery of microorganisms that are in small numbers in the sample. It is also an indicator for comparison with Mitis salivarius and the presence of other microorganisms in the sample.

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Components

gr/Lit	Components
60	Pancreatic Digest of Casein
9	Proteose Peptone No. 3
5	Proteose Peptone
1	Dextrose
50	Saccharose
4	Dipotassium Phosphate
0.075	Trypan Blue
0.0008	Crystal Violet
15	Agar
0.2 ± 7	pH

Quality Control

Dehydrated Appearance: Bluish-beige, free-flowing, homogeneous.
Prepared Appearance: Deep royal blue, slightly opalescent
Cultural response was observed under 5-10% CO2 at 35 ± 2°C for 18-48 hours.

توضیحات

Preparation

Shake the container well to homogenize the powder. Suspend 90 g of the powder in 1 L of purified water.
Autoclave at 121°C for 15 minutes.
Let the medium cool to a temperature of 55-50 degrees Celsius, then add 1 milliliter of 1% potassium tellurite solution (iS47083).
After adding potassium tellurite solution, do not heat the medium under any circumstances.

STORAGE

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

Test

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/Lit	Components
60	Pancreatic Digest of Casein
9	Proteose Peptone No. 3
5	Proteose Peptone
1	Dextrose
50	Saccharose
4	Dipotassium Phosphate
0.075	Trypan Blue
0.0008	Crystal Violet
15	Agar
0.2 ± 7	pH

Quality Control

Dehydrated Appearance: Bluish-beige, free-flowing, homogeneous.
Prepared Appearance: Deep royal blue, slightly opalescent
Cultural response was observed under 5-10% CO2 at 35 ± 2°C for 18-48 hours.

Mitis Salivarius Agar

Preparation

STORAGE

Test

Quality Control

Preparation

STORAGE

Test

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

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CoA & MSDS files

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