Preparation
- Add 5 milliliters of distilled water to the selective CNA supplement vial and mix gently until fully dissolved.
- Sterilize the solution using a filter.
- Add the prepared supplement along with 5-7 % sterile defibrinated horse blood to 500 ml of sterile cooled to 50 degree Celsius Columbia Blood Agar (i23046) culture medium and mix well.
- Mix well and pour into sterile Petri dishes.
STORAGE:
- Keep away from light.
- -20 degree Celsius.