Thermoacidurans Agar

Application: Thermoacidurans agar culture medium is used to culture and isolate the bacterium Bacillus thermoacidurans (Bacillus coagulans) from food materials.

Standards: ISIRI 2326, APHA, National standard of Iran

Catalogue Number: i23132

توضیحات

Preparation

  • Shake the container containing the Themoacidurans Agar culture medium powder well. Dissolve 39.2 grams of the medium in 1 liter of distilled water.
  • Boil until it is completely dissolved.
  • Autoclave at 121 degrees Celsius for 15 minutes.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Test

Test

  • Heat-shock the desired sample and add 1 milliliter of it to the plate.
  • Add 15-20 milliliters of cooled culture medium (45-50 degrees Celsius) to the plate.
  • Gently rotate the plate to mix the sample and the medium well.
  • Incubate the plates at 55±1°C for 3-5 days.

Results and Interpretation

After incubation, examine the plates for colony morphology and number.

Note

  • Other bacteria may also grow on this medium besides Bacillus themoacidurans. To ensure the type of bacteria, perform microscopic and biochemical tests.
پرسش و پاسخ

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Components
gr/Lit Components
5.0t
Proteose Peptone
5.0t
Yeast Extract
5.0
Dextrose
4.0t
Dipottassium Phosphate
0.2t
Manganese Sulfate
20.0t
Agar
0.2 ± 5.0t
Final pH
Quality Control

Quality Control

  • Dehydrated Appearance: Light tan, free-flowing, homogeneous.
  • Prepared Appearance: Light amber, opalescent
  • Cultural Response after inoculation and incubate at 55 ± 1°C for 18-48 hours.
Growth ATCC Standard Strain
Good
8038
Bacillus thermoacidurans
توضیحات

Preparation

  • Shake the container containing the Themoacidurans Agar culture medium powder well. Dissolve 39.2 grams of the medium in 1 liter of distilled water.
  • Boil until it is completely dissolved.
  • Autoclave at 121 degrees Celsius for 15 minutes.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

Test

Test

  • Heat-shock the desired sample and add 1 milliliter of it to the plate.
  • Add 15-20 milliliters of cooled culture medium (45-50 degrees Celsius) to the plate.
  • Gently rotate the plate to mix the sample and the medium well.
  • Incubate the plates at 55±1°C for 3-5 days.

Results and Interpretation

After incubation, examine the plates for colony morphology and number.

Note

  • Other bacteria may also grow on this medium besides Bacillus themoacidurans. To ensure the type of bacteria, perform microscopic and biochemical tests.
پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components
gr/Lit Components
5.0t
Proteose Peptone
5.0t
Yeast Extract
5.0
Dextrose
4.0t
Dipottassium Phosphate
0.2t
Manganese Sulfate
20.0t
Agar
0.2 ± 5.0t
Final pH
Quality Control

Quality Control

  • Dehydrated Appearance: Light tan, free-flowing, homogeneous.
  • Prepared Appearance: Light amber, opalescent
  • Cultural Response after inoculation and incubate at 55 ± 1°C for 18-48 hours.
Growth ATCC Standard Strain
Good
8038
Bacillus thermoacidurans

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen