Dichloran Glycerol (DG18) Agar Base

Application: The selective medium is used for isolation and enumeration of xerophilic molds in dry and semi-dry foods with low water activity, as well as for counting molds and yeasts in food products.

Standard: ISO 21527-2

Catalogue Number: i23056

توضیحات
  • Shake the container well before use. Dissolve 31.6 grams of the medium in one liter of distilled water.
  • Boil the solution to completely dissolve the powder.
  • Add 175 milliliters of glycerol (i880083) and mix well.
  • Autoclave at 121 degrees Celsius for 15 minutes.
  • After autoclaving, cool the medium to 50 degrees Celsius and pour it into the petri dishes.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

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Test

Test

  • Soaked the food sample in Peptone Water (i23137) culture medium and prepare a broth according to the relevant protocol.
  • Inoculate 0.1 to 0.5 milliliters of the sample onto the culture plate and spread it evenly over the surface according to the relevant protocol.
  • Incubate the inoculated plates for up to 6 days at 25°C.

Results and Interpretation

  • Check the growth on the plate after 4, 5, and 6 days of incubation at 25 degrees Celsius.
  • Calculate the number of colonies per 1 gram of food.

Related Product

Cat.Number Vial content (for 500 ml culture medium) Product
iS47076
187.5 mlt
Glycerol
Quality Control

Quality Control

  • Dehydrated Appearance: Homogeneous, free flowing, and beige.
  • Prepared Appearance: Prepared medium is trace to slightly hazy and beige.
  • Cultural Response after inoculation and incubate at 22-25°C for up to 5-7 days.
Growth ATCCt Standard Strain
Goodt
9763t
Saccharomyces cerevisiae
Negative
6633t
Bacillus subtilus
Negativet
25922t
Escherichia coli
Components
gr/Lit Components
5.0
Peptone
10.0
Glucose
1.0
Potassium Dihydrogen Phosphate
0.002
Dichloran
0.5
Magnesium Sulfate
0.1
Chloramphenicol
15.0
Agar
0.2 ± 5.6t
Final pH
توضیحات
  • Shake the container well before use. Dissolve 31.6 grams of the medium in one liter of distilled water.
  • Boil the solution to completely dissolve the powder.
  • Add 175 milliliters of glycerol (i880083) and mix well.
  • Autoclave at 121 degrees Celsius for 15 minutes.
  • After autoclaving, cool the medium to 50 degrees Celsius and pour it into the petri dishes.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Test

Test

  • Soaked the food sample in Peptone Water (i23137) culture medium and prepare a broth according to the relevant protocol.
  • Inoculate 0.1 to 0.5 milliliters of the sample onto the culture plate and spread it evenly over the surface according to the relevant protocol.
  • Incubate the inoculated plates for up to 6 days at 25°C.

Results and Interpretation

  • Check the growth on the plate after 4, 5, and 6 days of incubation at 25 degrees Celsius.
  • Calculate the number of colonies per 1 gram of food.

Related Product

Cat.Number Vial content (for 500 ml culture medium) Product
iS47076
187.5 mlt
Glycerol
Quality Control

Quality Control

  • Dehydrated Appearance: Homogeneous, free flowing, and beige.
  • Prepared Appearance: Prepared medium is trace to slightly hazy and beige.
  • Cultural Response after inoculation and incubate at 22-25°C for up to 5-7 days.
Growth ATCCt Standard Strain
Goodt
9763t
Saccharomyces cerevisiae
Negative
6633t
Bacillus subtilus
Negativet
25922t
Escherichia coli
Components
gr/Lit Components
5.0
Peptone
10.0
Glucose
1.0
Potassium Dihydrogen Phosphate
0.002
Dichloran
0.5
Magnesium Sulfate
0.1
Chloramphenicol
15.0
Agar
0.2 ± 5.6t
Final pH

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen