Kovac’s (Indole) Reagent

To identify the ability of microorganisms, especially Enterobacteriaceae, to separate indole from the tryptophan molecule using the tryptophanase enzyme.

Catalogue Number: iR95008

توضیحات

Test

Add 0.2-0.5 milliliters of the Kovac’s reagent to 5 milliliters of the 24-48 hour culture medium. You can use a bacterium grown in tryptone water culture medium.
The formation of a red ring on the surface of the culture medium indicates a positive indole test.

پرسش و پاسخ

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Components

	Composition
0.5 g	4-(Dimethylamino)benzaldehyde
7.5 ml	Butanol
2.5 ml	HCl (concentrated)

Quality Control

Appearance Yellow solution.
Clarity: Clear solution without any particles.
Cultural Response of 24 – 48 hour incubated culture (Tryptone Water – 123185) and adding 0.2 – 0.5 ml of Kovac’s Reagent.

Indole production	Microorganism (ATCC)
Negative (no red ring)	E. aerogenes (13048)
Positive (red ring at the interface of the medium)	E. coli (25922)

توضیحات

Test

Add 0.2-0.5 milliliters of the Kovac’s reagent to 5 milliliters of the 24-48 hour culture medium. You can use a bacterium grown in tryptone water culture medium.
The formation of a red ring on the surface of the culture medium indicates a positive indole test.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

	Composition
0.5 g	4-(Dimethylamino)benzaldehyde
7.5 ml	Butanol
2.5 ml	HCl (concentrated)

Quality Control

Appearance Yellow solution.
Clarity: Clear solution without any particles.
Cultural Response of 24 – 48 hour incubated culture (Tryptone Water – 123185) and adding 0.2 – 0.5 ml of Kovac’s Reagent.

Indole production	Microorganism (ATCC)
Negative (no red ring)	E. aerogenes (13048)
Positive (red ring at the interface of the medium)	E. coli (25922)

Kovac’s (Indole) Reagent

Test

Test

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

Downloading CoA & MSDS files

CoA & MSDS files

Downloading CoA & MSDS files