Preparation
- Add 5 mL of sterile distilled water to the vial and mix well.
- Add the prepared supplement to 500 ml of sterile and cooled Mueller-Kauffman Tetrathionate broth culture medium containing novobiocin (i23389) at a temperature of 50 degrees Celsius.
- Mix well and pour into sterile test tubes.
STORAGE:
- Keep away from light.
- -20 degree Celsius.