Preparation
- Shake the container of Clostridium Difficile Agar well.
- Suspend 69.1 g of the medium in 1 liter of purified water.
- Autoclave at 121°C for 15 minutes.
- Cool to 45 – 50°C.
- Aseptically add 7% horse blood, and contents of 2 vials of CCFA Supplement.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.