توضیحات
Preparation
- Shake the container of Columbia Blood Agar Base well.
- Dissolve 44 grams in 1 L of distilled water.
- Autoclave at 121°C for 15 minutes.
- Cool to 45 – 50 °C,
- For Blood Agar, introduce 5% v/v sterile defibrinated sheep blood to the sterile cooled base.
- For Chocolate Agar, add 10% v/v sterile defibrinated sheep blood to the sterile cooled base, and heat to 80°C for 10 minutes with constant agitation.
- For Brucella species: Mix the rehydrated contents of 1 vial of Brucella Selective Supplement with 500 ml of sterile molten base.
- For Campylobacter species: Combine the rehydrated contents of 1 vial of a Campylobacter Supplement (I, II, III, VI) with 500 ml of sterile molten base. Add rehydrated contents of 1 vial of Campylobacter Growth Supplement and 5-7% v/v horse or sheep blood.
- For Gardnerella species: Blend the rehydrated contents of 1 vial of G. Vaginalis Selective Supplement with 500 ml of sterile molten base.
- For Cocci: Mix the rehydrated contents of 1 vial of Staph-Strep Supplement or Strep Supplement or Streptococcus Selective Supplement with 500 ml of sterile molten base.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
