Corn Meal Agar

Application: This is a multi-purpose medium for cultivating fungi. Poly-sorbate 80 is also used to test the ability of Candida strains to produce chlamydospores.

Catalogue Number: i23050

توضیحات

Preparation

Shake the container containing the cornmeal agar culture medium powder well. Dissolve 17 grams of the culture medium powder in 1 liter of distilled water.
If necessary, add 1% poly-sorbate 80 (i880218) or 1% dextrose (i880081) and mix well.
Heat for 1 minute until the powder is completely dissolved in water.
Autoclave at 121°C for 15 minutes.

STORAGE:

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

Test

Take a colony from the surface of the SDA medium and create a deep groove in the cornmeal agar medium, like a horizontal slot.
Repeat this process for each colony.

Results and Interpretation

After 24-48 hours of incubation at a temperature of 25 ± 30 degrees Celsius, growth should be examined using a microscope.
Candida albicans produces clusters of round balls with mycelium of germinating cells and thick-walled chlamydospores.

Note

Organisms must be isolated in a suitable culture medium before cultivation for identification.
A cornmeal agar plate can be used to identify four or five different colonies of Candida that have grown on SDA.
Adding 0.001% (w/v) trypan blue to the culture medium provides a suitable background for better observation of morphological features.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Dehydrated Appearance: Cream to yellow, free-flowing, homogeneous.

Prepared Appearance: Amber, slightly opalescent.

Cultural characteristics were observed after incubation at 25-30°C for up to 4 days (up to 4 weeks if necessary).

Chlamydospores	Growth	ATCC	Organism
Positive	Good	10231	Candida albicans
Negative	Good	16404	Aspergillus brasiliensis
Negative	Good	9763	Saccharomyces cerevisiae

Components

gr/Lit	Components
2	Corn Meal infusion from (solids)
15	Agar
6 ± 0.2	Final pH

توضیحات

Preparation

Shake the container containing the cornmeal agar culture medium powder well. Dissolve 17 grams of the culture medium powder in 1 liter of distilled water.
If necessary, add 1% poly-sorbate 80 (i880218) or 1% dextrose (i880081) and mix well.
Heat for 1 minute until the powder is completely dissolved in water.
Autoclave at 121°C for 15 minutes.

STORAGE:

Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.

Test

Take a colony from the surface of the SDA medium and create a deep groove in the cornmeal agar medium, like a horizontal slot.
Repeat this process for each colony.

Results and Interpretation

After 24-48 hours of incubation at a temperature of 25 ± 30 degrees Celsius, growth should be examined using a microscope.
Candida albicans produces clusters of round balls with mycelium of germinating cells and thick-walled chlamydospores.

Note

Organisms must be isolated in a suitable culture medium before cultivation for identification.
A cornmeal agar plate can be used to identify four or five different colonies of Candida that have grown on SDA.
Adding 0.001% (w/v) trypan blue to the culture medium provides a suitable background for better observation of morphological features.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Quality Control

Dehydrated Appearance: Cream to yellow, free-flowing, homogeneous.

Prepared Appearance: Amber, slightly opalescent.

Cultural characteristics were observed after incubation at 25-30°C for up to 4 days (up to 4 weeks if necessary).

Chlamydospores	Growth	ATCC	Organism
Positive	Good	10231	Candida albicans
Negative	Good	16404	Aspergillus brasiliensis
Negative	Good	9763	Saccharomyces cerevisiae

Components

gr/Lit	Components
2	Corn Meal infusion from (solids)
15	Agar
6 ± 0.2	Final pH

Corn Meal Agar

Preparation

STORAGE:

Test

Test

Results and Interpretation

Note

Quality Control

Preparation

STORAGE:

Test

Test

Results and Interpretation

Note

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

CoA & MSDS files

Downloading CoA & MSDS files

CoA & MSDS files

Downloading CoA & MSDS files