- Shake the container well before use. Dissolve 31.6 grams of the medium in one liter of distilled water.
- Boil the solution to completely dissolve the powder.
- Add 175 milliliters of glycerol (i880083) and mix well.
- Autoclave at 121 degrees Celsius for 15 minutes.
- After autoclaving, cool the medium to 50 degrees Celsius and pour it into the petri dishes.
STORAGE:
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.