Dichloran Rose Bengal Chloramphenicol (DRBC) Agar

Dichloran Rose Bengal Chloramphenicol (DRBC) Agar

Application: This is a selective medium for enumeration of food spoilage fungi and yeast.

استاندارد: BAM, APHA, ISO 21527-1, ISIRI 1899-1 (Iran National Standard)

شماره کاتالوگ: i23058

توضیحات

Preparation

Thoroughly shake the DRBC Agar culture medium container. Dissolve 31.6 grams of the medium in 1 liter of distilled water.
Boil the solution until the medium powder is completely dissolved in water.
Autoclave the medium at 121 degrees Celsius for 15 minutes.
After autoclaving, cool the medium to 50 degrees Celsius and then pour it into the culture plate.

STORAGE:

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

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Test

Dilute the desired food sample according to the relevant protocol and prepare a serial dilution from it.
Place 0.1 mL of the sample on the agar plate and spread it completely over the surface.
Incubate inoculated plates in 25 degrees Celesius up to 5 days.

Results and Interpretation

Check the growth on the plate after 3, 4, and 4 days of incubation at 25 degrees Celsius.
Yeasts colonies on this culture medium appear pink due to the absorption of rose bengal.
Calculate the number of colonies per 1 gram of food.

Note

The DRBC culture medium has a low pH which can increase the likelihood of yeast growth inhibition in the presence of rose bengal. For this reason, if it is necessary to count yeast in addition to mold, it is better to use both Rose Bengal Chloramphenicol Agar (Catalog number i23159) and DRBC culture medium for culturing the sample simultaneously.
Due to the unique properties of DRBC Agar, it is possible that some fungal or yeast strains may not grow or show poor growth on this culture medium. Also, it is possible that some bacteria may not be completely or relatively inhibited from growing on this medium.
Keep in mind that DRBC Agar should not be exposed to light, as the rose bengal present in this medium is oxidized in the presence of light and produces toxic substances that are harmful to mold and yeast growth.

Realted Product

Cat. Number	packagingt	Product
i23159	100 gr - 500 gr	Rose Bengal Chloramphenicol Agar

Quality Control

Dehydrated Appearance: Pink, free-flowing, homogeneous.
Prepared Appearance: Pink
Cultural response after incubation in 25 degrees Celesius up to 5 days.

Growth	ATCCt	Standard Strain
Good	9763t	Saccharomyces cerevisiae
Good	10231	Candida albicans
Good	16404t	Aspergillus niger
Negativet	6633	Bacillus subtilus
Negative	25922	Escherichia coli

Components

gr/Lit	Components
5.0	Peptone
10.0	Glucose
1.0t	Potassium Dihydrogen Phosphate
0.002	Dichloran
0.5	Magnesium Sulfate
0.025	Rose Bengal
0.1	Chloramphenicol
15.0	Agar
0.2 ± 5.6t	Final pH

توضیحات

Preparation

Thoroughly shake the DRBC Agar culture medium container. Dissolve 31.6 grams of the medium in 1 liter of distilled water.
Boil the solution until the medium powder is completely dissolved in water.
Autoclave the medium at 121 degrees Celsius for 15 minutes.
After autoclaving, cool the medium to 50 degrees Celsius and then pour it into the culture plate.

STORAGE:

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Test

Dilute the desired food sample according to the relevant protocol and prepare a serial dilution from it.
Place 0.1 mL of the sample on the agar plate and spread it completely over the surface.
Incubate inoculated plates in 25 degrees Celesius up to 5 days.

Results and Interpretation

Check the growth on the plate after 3, 4, and 4 days of incubation at 25 degrees Celsius.
Yeasts colonies on this culture medium appear pink due to the absorption of rose bengal.
Calculate the number of colonies per 1 gram of food.

Note

The DRBC culture medium has a low pH which can increase the likelihood of yeast growth inhibition in the presence of rose bengal. For this reason, if it is necessary to count yeast in addition to mold, it is better to use both Rose Bengal Chloramphenicol Agar (Catalog number i23159) and DRBC culture medium for culturing the sample simultaneously.
Due to the unique properties of DRBC Agar, it is possible that some fungal or yeast strains may not grow or show poor growth on this culture medium. Also, it is possible that some bacteria may not be completely or relatively inhibited from growing on this medium.
Keep in mind that DRBC Agar should not be exposed to light, as the rose bengal present in this medium is oxidized in the presence of light and produces toxic substances that are harmful to mold and yeast growth.

Realted Product

Cat. Number	packagingt	Product
i23159	100 gr - 500 gr	Rose Bengal Chloramphenicol Agar

Quality Control

Dehydrated Appearance: Pink, free-flowing, homogeneous.
Prepared Appearance: Pink
Cultural response after incubation in 25 degrees Celesius up to 5 days.

Growth	ATCCt	Standard Strain
Good	9763t	Saccharomyces cerevisiae
Good	10231	Candida albicans
Good	16404t	Aspergillus niger
Negativet	6633	Bacillus subtilus
Negative	25922	Escherichia coli

Components

gr/Lit	Components
5.0	Peptone
10.0	Glucose
1.0t	Potassium Dihydrogen Phosphate
0.002	Dichloran
0.5	Magnesium Sulfate
0.025	Rose Bengal
0.1	Chloramphenicol
15.0	Agar
0.2 ± 5.6t	Final pH

Dichloran Rose Bengal Chloramphenicol (DRBC) Agar

STORAGE:

Test

Results and Interpretation

Note

Quality Control

STORAGE:

Test

Results and Interpretation

Note

Quality Control

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CoA & MSDS files

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