Preparation
- Shake the container of GC Medium well.
- Dissolve 7.2 grams of the medium in 100 ml of distilled water to create a double-strength base.
- Autoclave at 121°C for 15 minutes.
- Once sterilized, cool it down to 45 – 50°C
- and aseptically introduce separately prepared Haemoglobin in the form of a 100 ml sterile 2% solution and GC Supplement with Antibiotics.
- Mix well and pour into sterile Petri dishes.
- To enhance the medium’s selectivity, consider adding antibiotic supplements like Linco T Supplement, V.C.N. Supplement, V.C.N.T. Supplement, or Vanclo T Supplement.
- If improved nutritional properties are desired, incorporate Growth Supplement or Yeast Autolysate Supplement.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.