Preparation
- Add 2 ml of distilled water to the Helicobacter pylori selective supplement vial and mix gently until completely dissolved.
- Using a filter to sterilize it.
- Add the supplement to 500 mL of sterile cooled (to 50 degree Celsius) Columbia blood agar (i23046) under sterile conditions.
- Add 35 milliliters of horse blood to the medium.
- Mix well and pour into sterile Petri dishes.
STORAGE:
- Keep away from light.
- -20 degree Celsius.