توضیحات
Preparation
- Shake the container of Lowenstein Jensen Medium Base well.
- Suspend 37.5 g in 0.6 litre purified water, if required add 12 ml glycerol, mix thoroughly and
- Autoclave at 121°C for 15 minutes.
- Cool to about 50 °C,
- add 1 litre whole-egg homogenate prepared from fresh hen eggs under sterile conditions;
- stir to give a homogeneous mixture avoiding formation of bubbles.
- Dispense into sterile test tubes and allow to coagulate in a slant position by heating for 45minutes at 85 °C in an inspissator saturated with water vapour or in free-flowing steam.
- The culture medium should be heated once more in this way after about 24 hours to guarantee its sterility.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
