Methyl-red VOGES-PROSKAUER broth, MR-VP

Application: For the differentiation of bacteria based on acid production (Methyl Red test) and acetoin production (Voges-Proskauer reaction).

Industry: Clinical

Catalogue Number: i23249

توضیحات

Preparation

Shake the container of MR-VP Broth well.
Suspend 17 g of the powder in 1 L of distilled water. Mix well.
Distribute into tubes or flasks.
Autoclave at 121°C for 15 minutes.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

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Components

gr / L	Components
3.5	Pancreatic Digest of Casein
3.5	Peptic digest of Animal Tissue
5	Glucose
5	Potassium Dihydrogen Phosphate
0.2 ± 6.9	Final pH at 25°C

Quality Control

Dehydrated Appearance: Fine, homogeneous, free of extraneous material.
Prepared Appearance: Pale to light, yellow to tan, clear to slightly hazy.
Inoculate MR-VP Broth with growth from a single colony. Incubate at 35 ± 2°C for 48 hours Proceed with Methyl Red or Voges-Proskauer test.
Methyl Red Test: Transfer 2.5 mL of the MR-VP Broth culture to a tube (13 x 100mm). Add 5 drops of Methyl Red and observe for a color change.
Voges-Proskauer Test: Transfer 2.5 mL of the MR-VP Broth culture to a tube (13 x 100mm). Add 0.3 mL (6 drops) of Voges-Proskauer Reagent A (5% α-naphthol). Add 0.1 mL (2 drops) of Voges-Proskauer Reagent B (40% KOH). Gently agitate the tube and let stand for 10 – 15 minutes. Observe for a color change.

Voges-Prokauer	Methyl Red	Recovery	Organism (ATCC)
- (no change)	+ (red)	Good	Citrobacter freundii (8454)
+ (red)	- (yellow)	Good	Enterobacter aerogenes (13048)
- (no change)	+ (red)	Good	Escherichia coli (25922)
+	±	Serratia marcescens (14756)	Serratia marcescens (14756)

توضیحات

Preparation

Shake the container of MR-VP Broth well.
Suspend 17 g of the powder in 1 L of distilled water. Mix well.
Distribute into tubes or flasks.
Autoclave at 121°C for 15 minutes.

STORAGE

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr / L	Components
3.5	Pancreatic Digest of Casein
3.5	Peptic digest of Animal Tissue
5	Glucose
5	Potassium Dihydrogen Phosphate
0.2 ± 6.9	Final pH at 25°C

Quality Control

Dehydrated Appearance: Fine, homogeneous, free of extraneous material.
Prepared Appearance: Pale to light, yellow to tan, clear to slightly hazy.
Inoculate MR-VP Broth with growth from a single colony. Incubate at 35 ± 2°C for 48 hours Proceed with Methyl Red or Voges-Proskauer test.
Methyl Red Test: Transfer 2.5 mL of the MR-VP Broth culture to a tube (13 x 100mm). Add 5 drops of Methyl Red and observe for a color change.
Voges-Proskauer Test: Transfer 2.5 mL of the MR-VP Broth culture to a tube (13 x 100mm). Add 0.3 mL (6 drops) of Voges-Proskauer Reagent A (5% α-naphthol). Add 0.1 mL (2 drops) of Voges-Proskauer Reagent B (40% KOH). Gently agitate the tube and let stand for 10 – 15 minutes. Observe for a color change.

Voges-Prokauer	Methyl Red	Recovery	Organism (ATCC)
- (no change)	+ (red)	Good	Citrobacter freundii (8454)
+ (red)	- (yellow)	Good	Enterobacter aerogenes (13048)
- (no change)	+ (red)	Good	Escherichia coli (25922)
+	±	Serratia marcescens (14756)	Serratia marcescens (14756)

Methyl-red VOGES-PROSKAUER broth, MR-VP

Preparation

STORAGE

Quality Control

Preparation

STORAGE

Quality Control

ibresco | Producer of Microbial Culture Media | Antibiotics | Supplements

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CoA & MSDS files

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