Preparation
- Shake the container of mCCDA well.
- Suspend 22.75 g of the powder in 500 ml of purified water Mix well.
- Autoclave at 121°C for 15 minutes.
- Cool the medium to 45 – 50°C and
- Rehydrate content of 1 vial of CCDA Supplement in 5 ml distilled water, filter-sterilize it and add to the cooled medium.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.