Muller Kauffmann Tetrathionate-Novobiocin Broth – MKTTN

Application: For the selective enrichment of salmonellae from food and animal feed stuffs.

Industry: Water / Food

Catalogue Number: i23390

توضیحات

Preparation

  • Shake the container of Muller Kauffmann Tetrathionate-Novobiocin Broth well.
  • Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and
  • bring to the boil.
  • Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution
  • prepared by dissolving 25 g of potassium iodide in 10ml of water, adding 20 g of iodine and then diluting to 100ml with sterile water.
  • Do not autoclave the medium.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

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Components
gr / L Components
4.3
Peptic digest of Animal Tissue
8.6
Peptone from Casein
2.6
Sodium Chloride
38.7
Calcium Carbonate
30.5
Sodium Thiosulphate
4.78
Ox Bile
0.0096
Brillant Green
0.04
Novobiocin
0.2 ± 8.0
Final pH at 25°C
Quality Control

Quality Control

  • Dehydrated Appearance: Cream to greenish yellow, homogeneous, free-flowing.
  • Prepared Appearance: Light green colored opalescent solution forms with heavy white precipitate.
  • The medium was inoculated using the organisms listed below, then incubated for 20 – 24 hours at 37 °C.
Recovery Organism (ATCC)
None-poor
Escherichia coli (25922)
Inhibition
Shigella flexneri (12022)
Excellent
Salmonella Typhimurium (14028)
توضیحات

Preparation

  • Shake the container of Muller Kauffmann Tetrathionate-Novobiocin Broth well.
  • Suspend 89.5g of MKTTn in 1 litre of distilled water, mix well and
  • bring to the boil.
  • Cool to below 45°C. Immediately before use add 20ml of iodine-iodide solution
  • prepared by dissolving 25 g of potassium iodide in 10ml of water, adding 20 g of iodine and then diluting to 100ml with sterile water.
  • Do not autoclave the medium.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components
gr / L Components
4.3
Peptic digest of Animal Tissue
8.6
Peptone from Casein
2.6
Sodium Chloride
38.7
Calcium Carbonate
30.5
Sodium Thiosulphate
4.78
Ox Bile
0.0096
Brillant Green
0.04
Novobiocin
0.2 ± 8.0
Final pH at 25°C
Quality Control

Quality Control

  • Dehydrated Appearance: Cream to greenish yellow, homogeneous, free-flowing.
  • Prepared Appearance: Light green colored opalescent solution forms with heavy white precipitate.
  • The medium was inoculated using the organisms listed below, then incubated for 20 – 24 hours at 37 °C.
Recovery Organism (ATCC)
None-poor
Escherichia coli (25922)
Inhibition
Shigella flexneri (12022)
Excellent
Salmonella Typhimurium (14028)

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen