Preparation
- Add 5 mL of sterile distilled water to the vial and mix well to completely dissolved.
- Using a filter to sterilize it.
- Under sterile conditions, add the supplement to 500 mL of sterile and cooled Agar Palcam (i23212) culture medium at 50°C.
- Mix well and pour into sterile Petri dishes.
Note
- Acriflavine hydrochloride is activated by light and becomes a growth inhibitor for Listeria.
STORAGE:
- Keep away from light.
- -20 degree Celsius.