Preparation
- Shake the container of Peptone Water well.
- Dissolve 15 g of the powder in 1 L of purified water.
- Autoclave at 121°C for 15 minutes.
For Determining Carbohydrate Fermentation Patterns
- Add 1.8 mL 1% phenol red solution to 1 liter rehydrated Peptone Water. Mix well.
- Dispense into test tubes containing inverted Durham vials.
- Autoclave at 121°C for 15 minutes.
- Aseptically add sufficient sterile carbohydrate solution to yield a 1% final concentration. Rotate each tube to thoroughly distribute the carbohydrate.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.