توضیحات
Preparation
- Shake the container of Phenol Red Broth well.
- Dissolve 15 g of the powder in 1 L of purified water.
- Agar (0.5 – 1.0 g) may be added if it is desirable to minimize convection currents in the broth.
- Dispense in test tubes containing inverted Durham’s tubes.
- Autoclave at 121°C for 15 minutes.
- Tubes should be packed loosely to ensure free access of steam. Aseptically introduce a carbohydrate solution that has been filter-sterilized or autoclave-sterilized into the base medium.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
