Phenylalanine Agar

Application: Also known as Phenylalanine Deaminase Medium, is used for the differentiation of enteric Gram-negative bacilli on the basis of their ability to produce phenylpyruvic acid from phenylalanine by oxidative deamination.

Industry: Food

Catalogue Number: i23403

توضیحات

Preparation

  • Shake the container of Phenyl Alanine Agar well.
  • Suspend 23 g of the powder in 1 L of purified water. Mix well.
  • Autoclave at 121°C for 15 minutes.
  • Dispense in tubes for slant cultures.
  • After appropriate incubation of bacteria, ferric chloride reagent is added on the agar. Formation of a green color in 1 – 5 min indicates the production of phenylpyruvic acid.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

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Components
gr / L Components
2
DL-Phenylalanine
3
Yeast Extract
5
Sodium Chloride
1
Disodium Phosphate
12
Agar
0.2 ± 7.3
Final pH at 25°C
Quality Control

Quality Control

  • Dehydrated Appearance: Light tan, free-flowing, homogeneous.
  • Prepared Appearance: Light amber, slightly opalescent.
  • Inoculate slant tubes and incubate at 35 ± 2°C for 18 -24 hours. After appropriate growth, add 3 – 5 drops of a 10% aqueous solution of ferric chloride and gently rotate to loosen the growth. Read reactions after 1 – 5 minutes.
Color reaction Recovery Organism (ATCC)
-
Good
Enterobacter aerogenes (13048)
+ (Green)
Good
Proteus vulgaris (13315)
+ (Green)
Good
Providencia alcalifaciens (9886)
توضیحات

Preparation

  • Shake the container of Phenyl Alanine Agar well.
  • Suspend 23 g of the powder in 1 L of purified water. Mix well.
  • Autoclave at 121°C for 15 minutes.
  • Dispense in tubes for slant cultures.
  • After appropriate incubation of bacteria, ferric chloride reagent is added on the agar. Formation of a green color in 1 – 5 min indicates the production of phenylpyruvic acid.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components
gr / L Components
2
DL-Phenylalanine
3
Yeast Extract
5
Sodium Chloride
1
Disodium Phosphate
12
Agar
0.2 ± 7.3
Final pH at 25°C
Quality Control

Quality Control

  • Dehydrated Appearance: Light tan, free-flowing, homogeneous.
  • Prepared Appearance: Light amber, slightly opalescent.
  • Inoculate slant tubes and incubate at 35 ± 2°C for 18 -24 hours. After appropriate growth, add 3 – 5 drops of a 10% aqueous solution of ferric chloride and gently rotate to loosen the growth. Read reactions after 1 – 5 minutes.
Color reaction Recovery Organism (ATCC)
-
Good
Enterobacter aerogenes (13048)
+ (Green)
Good
Proteus vulgaris (13315)
+ (Green)
Good
Providencia alcalifaciens (9886)

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen