Preparation
- Add 5 mL of sterile distilled water to the vial. Mix gently until it is completely dissolved.
- Using a filter to sterilize it.
- Add the supplement to 500 mL of sterile and cooled PSB culture medium to a temperature of 50 degrees Celsius under sterile conditions.
- Mix well and pour into test tubes.
STORAGE:
- Keep away from light.
- -20 degree Celsius.