Selenite Broth, Selenite F Broth

Application: Selenite Broth (Selenite F Broth) is used as an enrichment medium for the isolation of Salmonella from feces, urine, water, foods and other materials of sanitary importance.

Industry: Food / Clinical

Catalogue Number: i23167

توضیحات

Preparation

  • Shake the container of Selenite Broth well.
  • Suspend 23 g / liter at room temperature. If the medium does not dissolve readily, heat briefly (max. 60 °C).
  • if the medium is to be stored for a longer period of time filter-sterilize, dispense into suitable containers.
  • DO NOT OVERHEAT THE MEDIUM.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

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Components
gr / L Components
5
Peptone from Casein
4
Lactose
4
Sodium Selenite
3.5
Dipotassium Hydrogen Phosphate
6.5
Potassium Dihydrogen Phosphate
0.2 ± 7.0
Final pH at 25°C
Quality Control

Quality Control

  • Dehydrated Appearance: Off-white, free-flowing, homogeneous.
  • Prepared Appearance: Very light amber, clear to very slightly opalescent, may have a slight precipitate.
  • Inoculate slant tubes and incubate at 35 ± 2°C for 18 -24 hours. After incubation, subculture onto MacConkey Agar plates and incubate plated media at 35 ± 2°C for 18 -24 hours.
Colonies on MacConkey Agar Recovery Organism (ATCC)
Pink with bile precipitate
Partial to complete inhibition
Escherichia coli (25922)
Colorless
Good
Salmonella enterica (14028)
Colorless
Fair to Good
Shigella sonnei (9290)
توضیحات

Preparation

  • Shake the container of Selenite Broth well.
  • Suspend 23 g / liter at room temperature. If the medium does not dissolve readily, heat briefly (max. 60 °C).
  • if the medium is to be stored for a longer period of time filter-sterilize, dispense into suitable containers.
  • DO NOT OVERHEAT THE MEDIUM.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components
gr / L Components
5
Peptone from Casein
4
Lactose
4
Sodium Selenite
3.5
Dipotassium Hydrogen Phosphate
6.5
Potassium Dihydrogen Phosphate
0.2 ± 7.0
Final pH at 25°C
Quality Control

Quality Control

  • Dehydrated Appearance: Off-white, free-flowing, homogeneous.
  • Prepared Appearance: Very light amber, clear to very slightly opalescent, may have a slight precipitate.
  • Inoculate slant tubes and incubate at 35 ± 2°C for 18 -24 hours. After incubation, subculture onto MacConkey Agar plates and incubate plated media at 35 ± 2°C for 18 -24 hours.
Colonies on MacConkey Agar Recovery Organism (ATCC)
Pink with bile precipitate
Partial to complete inhibition
Escherichia coli (25922)
Colorless
Good
Salmonella enterica (14028)
Colorless
Fair to Good
Shigella sonnei (9290)

CoA & MSDS files

Downloading CoA & MSDS files

 
 

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen

CoA & MSDS files

Downloading CoA & MSDS files

:To download the CoA file

Enter the LOT number printed on the container

:To download the MSDS file

Enter the catalog number (REF) printed on the container without the hyphen