توضیحات
Preparation
- Shake the container of Tetrathionate Broth Base well.
- Suspend 46 g in 1 litre of purified water,
- heat briefly to the boil and cool rapidly. DO NOT AUTOCLAVE.
- Prior to use, add 20 ml iodine/potassium iodide solution, if desired 10 ml of a 0.1 % brilliant green solution and if required 0.04 g novobiocin.
- Avoid any further heating.
- When dispensing the prepared medium, make sure that any precipitate formed is evenly suspended.
- For preparation of the iodine/potassium iodide solution mix Iodine 6 g, potassium iodide 5 g, distilled water 20 ml.
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.
