Preparation
- Shake the container well to homogenize the powder. Weigh 15 grams of the powder and dissolve it in 1 liter of distilled water.
- Warm slightly with frequent agitation to completely dissolve the powder.
- Autoclave at 121°C for 15 minutes.
For Determining Carbohydrate Fermentation Patterns
- Add 1.8 milliliters of a 1% Phenol Red solution (i880137) to one liter of Tryptone Water. Mix well.
- Dispense into test tubes containing inverted Durham vials. .
- Autoclave at 121°C for 15 minutes.
- Aseptically add sufficient sterile carbohydrate solution to yield a 1% final concentration. Rotate each tube to thoroughly distribute the carbohydrate.
STORAGE
Store dehydrated medium below 30 °C and prepared medium at 2-8 °C. The best time to use is before the expiration date printed on the product label.