Violet Red Bile Glucose Agar (VRBGA)

Application: Isolation and enumeration of enterobacteria in food samples.

Standards: ISIRI 2461-1, ISO (Iran National Standard)

Catalogue Number: i23193

توضیحات

Preparation

Shake the container of the VRBGA medium well. Dissolve 39.5 grams of the medium in 1 liter of distilled water.
Heat it until boiling and completely dissolved. Do not heat it for more than 2 minutes after that.
Do not autoclave the medium. Avoid overheating the medium.
After the medium has cooled, mix it well and pour it into the petri dishes.
Prepared plates can be stored for up to 2 weeks at 4°C, away from light.

STORAGE

The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.

Test

This medium is used in both the pour plate (with or without overlay) and spread methods.
Inoculate the sample into the culture medium according to your desired standard method.
Incubate at 35±2°C for 18-24 hours.

Results and Interpretation

Red colonies that have a dark halo (due to the precipitation of bile salts) around them are likely to be enterobacteria. For confirmation, select suspicious colonies for fermentation and oxidase tests.

Note

اگر از محیط VRBGA به روش پور پلیت استفاده می شود، باید تازه تهیه شود و تا ۴ ساعت پس از آن استفاده شود.

پرسش و پاسخ

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Components

gr/Lit	Components
7.0t	Peptone form Meat
3.0t	Yeast Extract
5.0t	Sodium Chloride
10.0t	D-glucose
1.5t	Bile Salt Mixture
0.03t	Neutral Red
0.002t	Crystal Violet
13.0t	Agar
0.2 ± 6.8t	Final pH

Quality Control

Dehydrated Appearance: Pink-beige to pink, free-flowing, homogeneous (may contain small dark particles).
Prepared Appearance: Reddish-purple, slightly opalescent.
Cultural characteristics were observed after incubation at 35 ± 2°C for 18- 24 hours.

Reaction	Growtht	ATCC	Standard Strain
Red to purple colonies, with bile precipitate	Goodt	25922t	Escherichia coli
Red to purple colonies, with bile precipitatet	Goodt	14028	Salmonella typhimurium
Colorless to red colonies, no bile precipitate	None to poort	25923t	Staphylococcus aureus
-	Nonet	29212	Enterococcus faecalis

توضیحات

Preparation

Shake the container of the VRBGA medium well. Dissolve 39.5 grams of the medium in 1 liter of distilled water.
Heat it until boiling and completely dissolved. Do not heat it for more than 2 minutes after that.
Do not autoclave the medium. Avoid overheating the medium.
After the medium has cooled, mix it well and pour it into the petri dishes.
Prepared plates can be stored for up to 2 weeks at 4°C, away from light.

STORAGE

Test

This medium is used in both the pour plate (with or without overlay) and spread methods.
Inoculate the sample into the culture medium according to your desired standard method.
Incubate at 35±2°C for 18-24 hours.

Results and Interpretation

Note

اگر از محیط VRBGA به روش پور پلیت استفاده می شود، باید تازه تهیه شود و تا ۴ ساعت پس از آن استفاده شود.

پرسش و پاسخ

شما نیز میتوانید سوالات خود را ثبت کنید!

اگر سوالی در مورد محصول دارید از این قسمت بپرسید!

Components

gr/Lit	Components
7.0t	Peptone form Meat
3.0t	Yeast Extract
5.0t	Sodium Chloride
10.0t	D-glucose
1.5t	Bile Salt Mixture
0.03t	Neutral Red
0.002t	Crystal Violet
13.0t	Agar
0.2 ± 6.8t	Final pH

Quality Control

Dehydrated Appearance: Pink-beige to pink, free-flowing, homogeneous (may contain small dark particles).
Prepared Appearance: Reddish-purple, slightly opalescent.
Cultural characteristics were observed after incubation at 35 ± 2°C for 18- 24 hours.

Reaction	Growtht	ATCC	Standard Strain
Red to purple colonies, with bile precipitate	Goodt	25922t	Escherichia coli
Red to purple colonies, with bile precipitatet	Goodt	14028	Salmonella typhimurium
Colorless to red colonies, no bile precipitate	None to poort	25923t	Staphylococcus aureus
-	Nonet	29212	Enterococcus faecalis

Violet Red Bile Glucose Agar (VRBGA)

Preparation

STORAGE

Test

Test

Results and Interpretation

Note

Quality Control

Preparation

STORAGE

Test

Test

Results and Interpretation

Note

Quality Control

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