Preparation
- Shake the container of YM Agar well.
- Suspend 41 g in 1 liter of distilled water and Mix well.
- Autoclave at 121°C for 15 minutes.
- To increase selectivity, acidify the medium to pH 3.0 to 4.0 by adding sterile 10% HCl, tartaric acid or 10% citric acid or add antibiotics (penicillin 20 units per mL final concentration or streptomycin 40 μμg per mL final concentration)
STORAGE
The medium powder should be kept tightly closed and stored at temperatures below 30 degrees Celsius, and the medium should be stored at a temperature of 2-8 degrees Celsius. The best time to use is before the expiration date printed on the product label.